Scenario in Order to Test the Susceptibility of Bacteria (If the the Bacteria

question 23

Multiple Choice

Scenario
In order to test the susceptibility of bacteria (if the bacteria are susceptible to being killed) to an antibiotic, a common laboratory test called the Bauer-Kirby Disk Diffusion test is used. First, a petri dish (a shallow, circular, transparent dish with a flat lid) is partially filled with nutrient-enriched agar (a gelatinous material) . The nutrients encourage growth of specific bacterial species. The agar is poured as liquid but then typically solidifies to a soft consistency. Once the agar is set, a "lawn" of bacteria is spread such that a thin layer covers the top of the agar. Antibiotics are then applied to the lawn in a small dose, often through small, saturated paper disks. The dish is typically incubated for 24-48 hours and then observed for susceptibility. If the antibiotic is effective, it will kill the bacteria and leave a clear area called the "zone of inhibition" due to inhibited bacterial growth. The area starts at the source of the antibiotic and radiates outward. Scientists measure the diameter of the zone and then compare it to an established "cutoff value" for a zone specific to antibiotic/organism combinations. A large zone of inhibition in comparison to the standard indicates susceptibility while a small or no zone indicates resistance. Consider the following image, and answer the questions that follow:
-What can you conclude about the susceptibility of the bacteria to the antibiotics?

• A)The bacteria are more susceptible to antibiotic A than antibiotic C.
• B)The bacteria are more susceptible to antibiotic D than antibiotic B.
• C)The bacteria are more susceptible to antibiotic C than antibiotic E.
• D)The bacteria are more susceptible to antibiotic D than antibiotic A.

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Definitions:

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