Site Directed Mutagenesis of the MS2 Genome Was Carried Out

question 5

Multiple Choice

Site directed mutagenesis of the MS2 genome was carried out to abolish the MJ stem structure that is just upstream of the start codon of the replicase gene.Which of the following describes the result of this experiment?

• A) The coat protein was unable to bind to the viral genome.
• B) The maturation protein was unable to bind to the stem-loop.
• C) The ribosome could no longer bind to the start codon for the replicase gene.
• D) The replicase protein was unable to bind to the viral genome.
• E) Translation of replicase gene increased in the absence of coat protein translation.

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