A Sample of Water Is Tested for Mutagens

question 13

Multiple Choice

A sample of water is tested for mutagens. The water is subjected to a process known as high pressure liquid chromatography which can separate compounds based on their different biochemical properties. As material leaves the HPLC column its absorbance is measured at UV light which is used to detect a variety of different compounds. These readings were recorded as a trace on a graph (top chart in the figure) . Fractions from the column are collected and then tested in an Ames assay (bottom chart in the figure) . In this assay there were 900 colonies with the solvent alone control. Which fractions contain mutagens?

• A) 7 and 16
• B) 3 and 10
• C) 3, 7, 10, and 16
• D) None of these fractions appear to contain a mutagen

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