[Solved] Real-time PCR can be used to quantify the original amount of RNA in a sample. What additional step must be accomplished in this particular application? A) careful design of specific forward and reverse primers B) RNA must first be converted to cDNA C) RNA must be denatured with formaldehyde to avoid secondary structures D) separation of mRNA from other RNA species E) none of the above Nature Symbolized: The representation of nature through symbols or motifs in art, literature, and culture to convey deeper meanings or messages. Photography: The art, practice, or process of creating images by recording light or other electromagnetic radiation, either electronically or chemically. Edward Weston: An influential American photographer known for his images of natural forms, nudes, and landscapes, pioneering a modern photographic aesthetic in the early to mid-20th century. Autobiographical: An autobiographical work is one where the author writes about their own life experiences, offering a personal perspective on events and reflections on their actions and choices.