In the Yeast Two-Hybrid Analysis Technique, in Vivo Protein-Protein Interactions

question 55

Multiple Choice

In the yeast two-hybrid analysis technique, in vivo protein-protein interactions are explored. The "bait" and "prey" proteins are translationally fused to the cleaved activation and DNA-binding domains of the GAL4 transcription factor. What is the target gene of GAL4?

• A) lacZ
• B) gadA/B
• C) GAL1
• D) all of the above
• E) none of the above

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Definitions:

VLTs

Video Lottery Terminals, a form of electronic gambling machine found in casinos and bars.

Exogenous Opiates

Narcotics, which bind to receptor sites throughout the body, including the brain, spinal cord, and bloodstream, and reduce the body’s production of endogenous opiates.

Opioids

Also known as narcotics. A class of central nervous system depressants whose main effects are the reduction of pain and sleep inducement. Opium, the alkaloid from which opioids are derived, comes from the seeds of the opium poppy, which is indigenous to Asia and the Middle East.

Heroin

A narcotic substance derived from morphine, originating from the seed pods of opium poppy plants, known for its strong addictive properties and illegality.