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You Have Amplified Four Unique Sequences Using PCR of the SSU

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Essay

You have amplified four unique sequences using PCR of the SSU rRNA gene from unknown microorganisms. Calculate the percent relatedness of each in comparison with the known sequence given to determine which strain(s) is/are most phylogenetically related. You have amplified four unique sequences using PCR of the SSU rRNA gene from unknown microorganisms. Calculate the percent relatedness of each in comparison with the known sequence given to determine which strain(s) is/are most phylogenetically related.


Definitions:

Heterozygous

Having two different alleles of a particular gene or genes, resulting in genetic variation.

Homozygous Dominant

An individual possessing two identical dominant alleles for a particular trait.

Monohybrid Cross

Cross between parents that differ in only one trait.

Phenotypic Ratio

The proportional relationship between the different phenotypes (observable traits) produced by a genetic cross, often expressed in simplest whole numbers.

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