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A defining commonality between biosafety risk group II handling techniques and a biosafety IV facility would be the need for:
Permafrost
Permanently frozen ground that occurs in high latitude or altitude regions.
Polar Grasslands
A cold, treeless region found in the high latitudes, often referred to as tundra, where the subsoil is permanently frozen and vegetation is sparse.
Arctic Tundra
A cold, treeless biome found in the Arctic regions, characterized by permafrost, low temperatures, and minimal vegetation.
Convection
The transfer of heat through a fluid (liquid or gas) caused by molecular motion.
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