The Melting Profile for a DNA Is Found to Differ

question 12

Multiple Choice

The melting profile for a DNA is found to differ in two different salt solutions.In 0.001 M NaCl,T_m = 55° C,and in 0.01 M NaCl,T_m = 65°C. The fundamental reason for this change is

• A) higher salt concentration increases the electrostatic repulsions between phosphates
• B) the base stacking is more effective in high salt
• C) hydrogen bonding between complementary bases in enhanced at high salt
• D) higher salt concentration decreases the electrostatic repulsions between phosphates

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Definitions:

SRS

Simple Random Sampling (SRS) is a method of selecting individuals from a population in such a way that every set of individuals has an equal chance of being chosen.

Sample Size

The number of observations or data points that constitute a sample drawn from a population for statistical analysis.

Standard Deviation

A measure of the amount of variation or dispersion in a set of values, showing how much each value in the set varies from the mean.

Binomial Populations

Populations for which the outcomes can be classified into two categories (success/failure) and the number of successes in a set number of trials can be predicted.