Exhibit 6A This Is a Reaction Going on in Your

question 57

Multiple Choice

Exhibit 6A This is a reaction going on in your muscle cells right this very minute:
The enzyme triose phosphate isomerase catalyzes this reaction in the forward direction as part of the glycolytic pathway.It follows simple Michaelis-Menten kinetics:
Typical cellular concentrations: triose phosphate isomerase = 0.1 nM
Dihydroxyacetone phosphate = 5 µM glyceraldehyde-3-phosphate = 2 µM
Refer to Exhibit 6A."Hindrate" is an inhibitor of triose phosphate isomerase.When it is added to cells at a concentration of 0.1 nM,the enzyme's K_M for the substrate is unchanged,but the apparent V_max is altered to 50 nM/sec.
In the following graph,which line best represents the Lineweaver-Burk plot obtained in the presence of hindrate?

• A) A
• B) B
• C) C
• D) D
• E) E

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