Exhibit 13A This Is a Map of the PBR322 Plasmid,numbers

question 13

Multiple Choice

Exhibit 13A This is a map of the pBR322 plasmid,numbers on the map refer to the base where the site is located.
Refer to Exhibit 13A.If a recombinant plasmid were obtained by inserting DNA into the EcoRV site,and the protein corresponding to the recombinant gene were expressed,which of the following statements would be false?

• A) The protein will probably contain some extra amino acids on its N-terminal.
• B) The plasmid will be resistant to ampicillin.
• C) The plasmid will not be able to replicate autonomously.
• D) The protein is not likely to be biologically active without some further treatment.
• E) The question cannot be answered, since it is unlikely that the protein will be expressed at all, because there is no promoter for mRNA synthesis.

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