Exhibit 13D The plasmid pSU922 is a circular DNA containing 25000 base pairs.The b-gal gene codes for the enzyme b-galactosidase,the product of which will turn bacterial colonies blue when grown in the presence of X-gal; the AmpR gene confers ampicillin resistance.
Refer to Exhibit 13D.Neglecting any discussion of whether it's a good or bad choice,I attempt to insert a gene fragment into the HindIII site and transform bacteria with the plasmid.How can I tell which transformants have the insert?
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