DrJohn Doe, a Research Microbiologist for a University Medical Center

question 29

Essay

Dr.John Doe, a research microbiologist for a university medical center, has been accused of sending Bacillus anthracis, the causative agent of anthrax, through the mail to the President.You, as a public health official with the CDC, have to determine if it is the same strain as the two strains of
A.Gram staining
B.Serological testing using agglutination
B.anthracis found cultured in his lab.Which method of identification would you use to compare these three bacterial samples?
C.Biochemical test identification
D.Pulse-field gel electrophoresis

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Definitions:

Biological Activity

The effect of a substance on living organisms, tissues, or cells, indicative of its potential therapeutic effects or toxicities.

Tertiary Structure

The three-dimensional shape of a protein molecule determined by interactions among its amino acids' side chains, beyond simple folding.

H-Bonds

Hydrogen bonds, a type of weak chemical bond that occurs when a hydrogen atom covalently bonded to one electronegative atom is also attracted to another electronegative atom.

Peptide Backbone

The main structural component of a peptide, consisting of a repeated sequence of amino acids linked by peptide bonds.