You Have Made a Specific DNA Probe That Will Bind

question 11

Multiple Choice

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work.Which of the following is a possible explanation for this negative result?

• A)You forgot to label the probe sequence with a reporter molecule.
• B)You forgot to add the mRNA to the DNA probe sample.
• C)You forgot to digest the probe with restriction endonucleases.
• D)You forgot to incubate the test at the 75^oC temperature required for hybridization.

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Definitions:

Room Temperature

The ambient air temperature considered comfortable for humans, typically around 20 to 22 degrees Celsius (68 to 72 degrees Fahrenheit).

Positioning

The act of placing or arranging someone or something in a particular posture or location, especially for medical or therapeutic purposes.

Rectal Sphincter Control

The ability to regulate the opening and closing of the rectal sphincter, critical for bowel movement control.

Dorsal Recumbent Position

A patient positioning where the individual lies on their back with legs bent and feet flat, often used for abdominal assessments.