The Criteria for an Adequate Separation for Isocratic Reverse-Phase HPLC

question 4

Multiple Choice

The criteria for an adequate separation for isocratic reverse-phase HPLC include which of the following?
I The retention factor for all peaks are in the range of 0.5 - 20.
II All peaks should be symmetric with an asymmetry factor B/A in the range of 0.9 - 15.
III For quantification,the minimum resolution between the two closest peaks should be 1.5,though 2.0 is best to compensate for small changes in separation conditions.
IV The pressure must be less than or equal to 15 MPa to prolong instrument life.

• A)I,II,III,and IV
• B)I,II,and IV
• C)I,II,and III
• D)II,III,and IV
• E)III and IV

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