Which Statement Is NOT True for Hydrophobic Interaction Chromatography

question 18

Multiple Choice

Which statement is NOT true for hydrophobic interaction chromatography?

• A)Hydrophobic interaction chromatography has a hydrophobic stationary phase,which repels water and is not wetted by water.The technique is used primarily for protein purification.
• B)Proteins are salted out of solution with ammonium,sodium,and potassium salts of phosphate and sulfate.Thiocyanate,iodide,and perchlorate salts increase the solubility of proteins in water.
• C)The proteins are eluted from the column with a gradient of increasing salt concentration,increasing the solubility of proteins in water.
• D)The most common stationary phase is an agarose gel with hydrophobic phenyl or alkyl groups attached and a pore size large enough to accommodate proteins.
• E)A protein sample with a large concentration of ammonium sulfate is applied to the column.The ammonium sulfate causes the protein to stick to the hydrophobic surface of the stationary phase.

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