____________________ Is Used to Isolate a Single Compound or Class

question 2

Multiple Choice

____________________ is used to isolate a single compound or class of compounds from a complex mixture by passing the sample through a column where only one solute is bound.The remaining solutes pass through the column.The bound solute is eluted from the column by changing a condition such as________________________________________ to weaken its binding.

• A)Adhesion chromatography;ratio of polar and nonpolar solvents
• B)Binding chromatography;pH or ionic strength
• C)Affinity chromatography;the polarity of the solvent
• D)Adhesion chromatography;the temperature of the column
• E)Affinity chromatography;pH or ionic strength