To See If Your Polymerase Chain Reaction Was Successful and It

question 12

Multiple Choice

To see if your polymerase chain reaction was successful and it amplified the right sequence of interest,you electrophorese the products from the reaction on an agarose gel.After staining the gel you find there are two bands of different sizes.Which one of the following is a possible explanation for these results?

• A)The PCR induced a frame shift mutation into the DNA sequence.
• B)RNA polymerase copied the DNA into two copies of RNA.
• C)The primers annealed to two different templates.
• D)a and b

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Definitions:

Hypothetical Constructs

Abstract concepts or theories that are utilized to describe or explain behaviors and phenomena, but which are not directly observable.

Attitudes

A learned tendency to evaluate things in a certain way, which can include people, issues, objects, or events.

Values

Core beliefs or principles that guide an individual's behavior and decision-making, reflecting what is important to them.

Affective Component

The emotional aspect of an attitude or response, reflecting feelings or emotions associated with a particular object, person, or situation.