In the Large-Scale Production of a Particular Human Protein in E.coli

question 40

Multiple Choice

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

• A)facilitate transfer of the cDNA into the E.coli cells.
• B)provide a promoter for the transcription of the cDNA in E.coli.
• C)facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms.
• D)prevent degradation of the expressed protein by E.coli proteases.

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Definitions:

Price Elasticity

A measure of how much the quantity demanded of a good responds to a change in its price, with higher elasticity indicating a greater responsiveness.

Profit-Maximizing Price

The optimal selling price for a product or service that leads to the highest possible profits for the producer or seller.

Weebles

A line of children's toys designed with a weighted bottom so that they wobble but don't fall down.

Market

A social or economic system where goods and services are exchanged between buyers and sellers using money or barter.