In the Large-Scale Production of a Particular Human Protein in E.coli

question 40

Multiple Choice

In the large-scale production of a particular human protein in E.coli cells,the cDNA corresponding to the protein was modified so that the expressed protein would have six histidine residues at the C-terminus.The purpose of this modification was to

• A)facilitate transfer of the cDNA into the E.coli cells.
• B)provide a promoter for the transcription of the cDNA in E.coli.
• C)facilitate purification of the expressed protein though binding to an affinity column containing chelated nickel atoms.
• D)prevent degradation of the expressed protein by E.coli proteases.

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Definitions:

Price Floor

A government-imposed minimum price charged for a commodity, aimed at preventing prices from falling too low.

Surplus

In economic terms, it refers to the amount by which production, assets, or resources exceed the portion used, often leading to excess supply in markets.

Shortage

A condition where the market's demand for a good or service outstrips its supply.

Price System

An economic mechanism wherein prices act as signals for the allocation of resources, influencing both producers and consumers in their decisions.