In Immunofluorescence Studies You Always See the Small G-Protein Ras

question 25

Multiple Choice

In immunofluorescence studies you always see the small G-protein Ras in a specific region of the cell and,given its protein sequence along with many other proteins localized to this area,you hypothesize that their C-terminal Cys-Ala-Ala-X (X can be any amino acid) motifs may be responsible for targeting these proteins to this subcellular region.To test this hypothesis,you genetically engineer the sequence encoding this motif onto a cDNA encoding green fluorescent protein.When immunofluorescence is used,where specifically would you expect to see GFP localized in the cell?

• A)in the nucleus
• B)in the cytoplasm
• C)in the Golgi
• D)at the plasma membrane

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