"3C" or Chromosome Conformation Capture Methods,used to Determine the Three-Dimensional

question 19

Multiple Choice

"3C" or chromosome conformation capture methods,used to determine the three-dimensional spatial organization of chromatin within nuclei of interphase cells,rely on a series of steps where the end result is the sequence analysis of purified DNA fragments.Which one of the following presents the correct order of steps you as an investigator need to follow in a 3C method strategy?

• A)shear DNA to 200-600 bp;cross-link proteins and DNA with formaldehyde
• B)ligate linkers marked with biotin onto DNA fragments;dilute and ligate the fragments
• C)cross-link streptavidin to DNA;purify and shear biotin-labeled fragments
• D)none of the above

Recognize dynamic and evolving aspects in descriptions.

Illustrate the importance of precise word choice in conveying clear messages.

Enhance vocabulary selection for specific contexts.

Improve sentence structuring for clearer communication.

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Definitions:

Normal Application

In cost accounting, it pertains to the standard method used to allocate overhead costs to products or services.

Reality

The state of things as they actually exist, as opposed to an idealistic or notional idea of them.

Process

A series of actions or steps taken in order to achieve a particular end.

Favorable Variance

The difference between actual results and expected (budgeted) results that indicates more efficient or cost-effective performance.