"3C" or Chromosome Conformation Capture Methods,used to Determine the Three-Dimensional

question 19

Multiple Choice

"3C" or chromosome conformation capture methods,used to determine the three-dimensional spatial organization of chromatin within nuclei of interphase cells,rely on a series of steps where the end result is the sequence analysis of purified DNA fragments.Which one of the following presents the correct order of steps you as an investigator need to follow in a 3C method strategy?

• A)shear DNA to 200-600 bp;cross-link proteins and DNA with formaldehyde
• B)ligate linkers marked with biotin onto DNA fragments;dilute and ligate the fragments
• C)cross-link streptavidin to DNA;purify and shear biotin-labeled fragments
• D)none of the above

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Definitions:

Amino Acids

Organic compounds that combine to form proteins, serving as the building blocks of life.

Codons

Sequences of three nucleotides in mRNA that specify the addition of a specific amino acid or signal the termination of protein synthesis.

DNA Replication

The biological process of producing two identical replicas of DNA from one original DNA molecule.

X-linked Recessive

An X-linked recessive trait is a mode of genetic inheritance by which a mutation in a gene on the X chromosome causes the phenotype to be expressed in males and in females who are homozygous for the gene mutation.