A Researcher Proposes That the Relationship Between a Particular Bacterial

question 23

Multiple Choice

A researcher proposes that the relationship between a particular bacterial species that produces a fat-soluble vitamin is mutualistic with its insect host.Which of the following procedures would best test the researcher's hypothesis?

• A)Determine which antibiotic kills the bacteria without harming the host,then administer this antibiotic to the insect and compare its growth with and without the vitamin
• B)Determine which insecticide kills the insect without harming the bacterial host,then compare vitamin production by the bacteria from insects treated with the insecticide to those from untreated insects
• C)Design a method to isolate the bacteria from the insect,then mutate the bacteria in the region of DNA that controls vitamin production and compare growth of the mutant and wild-type bacteria on minimal media
• D)Extract the DNA from the bacteria and from the insect,then use reverse-transcriptase PCR and primers specific to the gene that controls vitamin synthesis to compare transcription of RNA in both organisms

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Definitions:

Stat

An abbreviation for the term "statim," meaning immediately or without delay, often used in medical contexts.

Medication Administration

The process of providing a single dose of medication to a patient via any number of routes such as orally, intravenously, or intramuscularly.

Upright

In a vertical position; standing or sitting straight up.