A Molecular Biologist Is Interested in Purify a Recombinant Protein

question 28

Multiple Choice

A molecular biologist is interested in purify a recombinant protein by His-tagging,but the protein and vector both lack histidine residues.In this case,the molecular biologist could use which of the following technique(s) to acheive purification?

• A)Add a 6xHis-tag to the C-terminus of the protein.
• B)Add a series of histidine residues to the N-terminus of the protein.
• C)Amplify the histidine-encoding sequence by PCR and add it to the gene of interest.
• D)All of the choices are correct.

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Definitions:

Underfunded

Lacking sufficient financial resources or investment.

Stereotype Threat

The effect of a negative stereotype about one’s self upon one’s own test performance.

Inequality

The state of not being equal, especially in status, rights, and opportunities; discrepancies in resources and power within a society.

Culturally Specific

Pertaining to or characteristic of a particular culture, indicating that certain practices, beliefs, or preferences are unique to that culture.