You and a Friend Are Student Assistants in a Research

question 5

Multiple Choice

You and a friend are student assistants in a research laboratory that investigates the anti-cancer properties of proteins isolated from marine organisms.Your friend mentions that she is using a BAC vector to insert shark DNA into Escherichia coli,but after repeated attempts,has found that the bacterial cells fail to synthesize the encoded protein.What is your advice?

• A)If the shark DNA is unmodified,it contains introns that are not recognized by bacteria,therefore protein synthesis will not occur.
• B)Since shark DNA is eukaryotic,the cloning vector should be a YAC derived from yeast rather than a BAC derived from bacteria.
• C)It is impossible to clone eukaryotic DNA into a bacterial host,since eukaryotic DNA has introns and prokaryotic DNA does not.
• D)Because Escherichia coli is not naturally competent,it cannot serve as the cloning host for foreign DNA.