Which of the Following Techniques Exemplifies Phenotypic Rescue in a Host

question 67

Multiple Choice

Which of the following techniques exemplifies phenotypic rescue in a host cell?

• A)Introducing a genomic library from a cell that produces a particular gene product into a mutant host cell auxotroph that cannot synthesize the product.
• B)Introducing a genomic library from a mutant cell auxotroph for a particular gene product into a host cell that can synthesize that product constitutively.
• C)Removing genes that encode a particular gene product from a cell that expresses that product constitutively before creating a genomic library.
• D)Replacing a mutant gene with a functional gene in a DNA library from an auxotrophic mutant cell prior to introducing the library into a related host cell.

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Definitions:

Quasi-Experimental Design

A research design that attempts to establish a cause-and-effect relationship but does not employ random assignment to conditions, making it less strict than a true experimental design.

Random Assignment

The process of allocating participants randomly to different groups in an experiment to ensure each group is similar at the start.

Independent Variables

The variables in an experiment that are manipulated by the researcher to explore their effect on dependent variables.

True Experimental Design

A research design that includes random assignment of participants to groups and manipulation of an independent variable to determine its effect on a dependent variable.