If You Forgot to Heat Fix a Smear Before Doing

question 67

Multiple Choice

If you forgot to heat fix a smear before doing a Gram stain,which of the following might occur?

• A)The stains would not adhere to the bacteria.
• B)The smear may not adhere to the slide.
• C)The decolorization step of the Gram stain would not work properly.
• D)Gram-positive and Gram-negative bacteria would both stain purple.