You Have Made a Specific DNA Probe That Will Bind

question 11

Multiple Choice

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae, allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However, upon testing it out against a known Neisseria gonorrhoeae culture, you find that it does not work.Which of the following is a possible explanation for this negative result?

• A)You forgot to label the probe sequence with a reporter molecule.
• B)You forgot to add the mRNA to the DNA probe sample.
• C)You forgot to digest the probe with restriction endonucleases.
• D)You forgot to incubate the test at the 75^oC temperature required for hybridization.

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Definitions:

Mexican War

A conflict between the United States and Mexico from 1846 to 1848, resulting in the U.S. acquiring territories that include present-day California, Arizona, and New Mexico.

Tejanos

Residents of Mexican heritage living in Texas, especially those who were in the region before it became part of the United States.

Texas Borderland

Refers to the geographic area along the border between Texas and Mexico, characterized by its distinct cultural, social, and economic interactions.