You Have Made a Smear of a Bacterial Culture and Have

question 64

Multiple Choice

You have made a smear of a bacterial culture and have performed the Gram stain on it.Looking at the organism under the microscope, you notice that the cells do not seem to be the dark blue-purple of a gram-positive reaction, but instead are light purple.Your staining procedure was performed correctly.What is your best explanation as to why the bacteria have stained this way?

• A)The bacteria were taken from an inappropriate medium, and a chemical is interfering with proper staining of the cells.
• B)There is something in the wall of the bacteria that has affected the uptake of the crystal violet into the cell wall, thereby staining it improperly.
• C)The specimen did not undergo heat fixation before staining; therefore, the primary dye does not stick properly to the wall.
• D)These are mutant bacteria which cannot be stained like other bacteria.

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Definitions:

Loveless Marriages

Marital relationships lacking in affection and emotional connection between the partners.

Sexual Harassment

Unwelcome sexual advances, requests for sexual favors, and other verbal or physical conduct of a sexual nature that affects a person’s employment or creates a hostile environment.

Workplace Environment

The physical and psychological conditions under which employees work, including factors like culture, safety, and interpersonal relationships.

Moral

Relating to principles of right and wrong behavior and the goodness or badness of human character.