What DNA Sequencing Technique Is Useful for High Throughput Sequencing

question 19

Multiple Choice

What DNA sequencing technique is useful for high throughput sequencing?

• A)Sequencing using oligoarrays and fluorescent dyes.
• B)Dideoxynucleotides and radioactive nucleotides.
• C)Chemical degradation with radioactive nucleotides.
• D)Fluorescent nucleotides combined with acrylamide gel electrophoresis to separate the fragments.

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Definitions:

Phenotypes

The observable physical or biochemical characteristics of an organism, as determined by both genetic makeup and environmental influences.

Linkage Group

A set of genes located close together on the same chromosome that tend to be inherited together.

Chromosome

A chromosome is a long, continuous thread of DNA that contains numerous genes and regulatory elements, housed within the nucleus of every cell.

Testcross

Method of determining the genotype of an individual with a dominant phenotype: a cross between the individual and another individual known to be homozygous recessive.