Your Research Project Involves the Characterization of a Recently Identified

question 36

Multiple Choice

Your research project involves the characterization of a recently identified transcription factor.As part of your project,you want to determine if this transcription factor binds directly to any of the general transcription factors.Unfortunately,however,you are having trouble expressing and purifying the full length protein.Which of the following options may help you achieve your research goal?

• A)Since transcription domains cannot be separated,you will need to abandon this experiment and try a vastly different approach to your question.
• B)Express large amounts of the general transcription factors.
• C)Since transcription domains can be separated,try to express and purify only the DNA-binding domain.
• D)Since transcription domains can be separated,try to express and purify only the activation domain.

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