You Are Conducting a Genetic Screen to Isolate Nutritional Mutants

question 90

Multiple Choice

You are conducting a genetic screen to isolate nutritional mutants in yeast.Specifically,you want to isolate a histidine leucine double mutant.You start with a wild type yeast strain and mutagenize it with a UV light.Which of the following outlines the remaining steps for isolating such a mutant?

• A)Grow mutagenized yeast on rich mediA.Grow yeast on minimal media,as well as on minimal media containing histidine,minimal media containing leucine,and minimal media containing both histidine and leucine.Select for yeast that do not grow on minimal media,do not grow on media supplemented with only histidine or leucine,but do grow on minimal media supplemented with both histidine and leucine.
• B)Grow mutagenized yeast on minimal media.Grow yeast on rich media,as well as on minimal media containing histidine,minimal media containing leucine,and minimal media containing both histidine and leucine.Select for yeast that do not grow on rich media,do not grow on media supplemented with only histidine or leucine,but do grow on minimal media supplemented with both histidine and leucine.
• C)Grow mutagenized yeast on rich media.Grow yeast on minimal media,as well as on minimal media containing histidine,minimal media containing leucine,and minimal media containing both histidine and leucine.Select for yeast that grow on minimal media,grow on media supplemented with only histidine or leucine,but do not grow on minimal media supplemented with both histidine and leucine.
• D)Grow mutagenized yeast on minimal media.Grow yeast on rich media,as well as on minimal media containing histidine,minimal media containing leucine,and minimal media containing both histidine and leucine.Select for yeast that grow on rich media,grow on media supplemented with only histidine or leucine,but do not grow on minimal media supplemented with both histidine and leucine.

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