In a FISH Experiment,what Would Happen If Unbound Probe Was

question 21

Multiple Choice

In a FISH experiment,what would happen if unbound probe was not washed off?

• A)Nothing-it's not necessary to wash off the unbound probe.
• B)You would get false-positive results in different areas where the probe hadn't actually bound,but it was still sitting there and lighting up.
• C)Your FISH would be floating at the top of the tank due to the toxicity of the probe building up within them.
• D)Nothing-the target nucleotide sequences are labeled,not the probe.Therefore,excess unbound probe wouldn't matter for the experiment.

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