Cells Are Often Ground Up with Abrasive to Get to Their

question 22

Multiple Choice

Cells are often ground up with abrasive to get to their internal enzymes/molecules,followed by removal of the abrasives.Here are three situations and three outcomes.What is the correct interpretation of the results? In situation #1,radioactive amino acids are added to cell material,and radioactive proteins are produced.In situation #2,radioactive amino acids,cell material,and the enzyme DNAse (degrades DNA) are added together.Radioactive proteins are still produced.In situation #3,the ground-up cell material is allowed to sit for 24 hours before radioactive amino acids and DNAse are added to it.No radioactive protein is produced.What is the most likely interpretation?

• A)RNA polymerase has no activity after 24 hours,so no mRNA is transcribed and translated into radioactive proteinsin the last scenario.
• B)The radioactivity in the amino acids is altering/degrading the tRNA molecules,leading to no protein production in the last scenario.
• C)Natural RNAses present in the ground-up material will degrade any existing mRNAs in that 24-hour interval.Added DNAses breakdown DNA so that new RNAs are not synthesized.This will lead to a loss of capability to translate protein in the last scenario.
• D)The results are not interpretable from the information given.

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Definitions:

Research Hypothesis

A statement predicting the relationship between variables, formulated to be tested by research.

Null

Typically refers to the null hypothesis in statistical analysis, which posits that there is no significant difference or relationship between specified populations, any observed difference being due to sampling or experimental error.

Assume

To accept something to be true without question or proof.

Research Hypothesis

A specific, testable prediction about the relationship between two or more variables that a researcher aims to investigate.