Why Is Heating a First Step in PCR Amplification of Extracted

question 111

Multiple Choice

Why is heating a first step in PCR amplification of extracted DNA?

• A) The enzyme DNA polymerase works at a higher temperature.
• B) The nucleotides bind together at high heat.
• C) It is necessary for DNA strands to anneal to each other.
• D) It permits hydrogen bonds between DNA strands to dissociate.
• E) It is required for primers to bind permanently to the DNA.

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