Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

question 56

Multiple Choice

Why must double-stranded DNA probes be denatured when used in Northern blot experiments?

• A) To ensure that they are properly radioactively labeled or tagged with a dye.
• B) To ensure that the DNA coding strand hybridizes to RNA on the membrane.
• C) To ensure that both DNA strands bind the single-stranded RNA on the membrane.
• D) All of the above.
• E) None of the above.

---

Definitions:

Near-Death Experiences

Phenomena reported by some individuals who have been close to death but survived, often involving a sense of detachment from the body and visions.

Endorphins

Biochemical substances in the human body that act on the nervous system to reduce pain and boost pleasure, resulting in feelings of well-being.

Cardiac Arrest

A sudden loss of blood flow resulting from the failure of the heart to effectively pump.

Dopamine

A neurotransmitter involved in controlling movement and the reward centers in the brain.