The Colorless Molecule O-Nitrophenyl Galactoside (ONPG) Can Be Used as an Artificial

question 28

Multiple Choice

The colorless molecule o-nitrophenyl galactoside (ONPG) can be used as an artificial substrate in assays of $\beta$ -gal in E. coli extracts. Which of the following statements is NOT true with respect to the use of ONPG?

• A) Hydrolysis of ONPG by \(\beta\)-gal produces yellow o-nitrophenol.
• B) o-nitrophenol can be measured with a spectrophotometer.
• C) Hydrolysis of ONPG by \(\beta\)-gal also releases colorless galactose.
• D) The o-nitrophenol moiety is blue and more suitable for use in solid media.
• E) None of the above.

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