Why Must Double-Stranded DNA Probes Be Denatured When Used in Northern

question 56

Multiple Choice

Why must double-stranded DNA probes be denatured when used in Northern blot experiments?

• A) To ensure that they are properly radioactively labeled or tagged with a dye.
• B) To ensure that the DNA coding strand hybridizes to RNA on the membrane.
• C) To ensure that both DNA strands bind the single-stranded RNA on the membrane.
• D) All of the above.
• E) None of the above.

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