You Have Just Isolated Four Homologous Sequences Through PCR Amplification

question 37

Essay

You have just isolated four homologous sequences through PCR amplification of specific glycolysis protein genomic sequence from four separate bacterial cultures that you proved to be Escherichia coli through morphological and biochemical laboratory analysis. Unknown #3 looks different from the other three. Is this still an Escherichia coli? How can you explain this difference and what can you deduce about the gene product in comparison with the other three samples?
Unknown #1: A A T T T A C G A T A A A T G G A A A A A T T T C G A T
Unknown #2: A A T T T A C G A T A A A T G G A A A A A T T T C G A T
Unknown #3: A A T G G C C G G C G C G C G G A A A A A T T T C G A T
Unknown #4: A A T T T A C G A T A A A T G G A A A A A T T T C G A T

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Definitions:

Phagocytosis

A cellular process where cells engulf and digest large particles or microorganisms, an important feature of the immune response.

Chemotaxis

The movement of an organism or cell toward or away from a chemical stimulus.

White Blood Cell

A type of immune cell that is vital in the body's defense against infection.

Injury Site

The location on the body where an injury has occurred.