You Cut Some Sheep DNA and Some Plasmid DNA with the Same

question 3

Multiple Choice

You cut some sheep DNA and some plasmid DNA with the same restriction enzyme.The plasmid contains an Amp^R gene and a lacZ gene.You mix together the fragments of sheep DNA and the linearized plasmid.You transform bacteria with your mixture,and plate the bacterial cells on growth media containing the antiobiotic ampicillin and X-gal (the substrate of beta-galactosidase) .You need to pick the bacterial colonies that contain recombinant plasmids.Which colonies do you choose?

• A)only the blue colonies - these contain recombinant plasmids
• B)only the white colonies - these contain recombinant plasmids
• C)the largest colonies - these are the most resistant to ampicillin and thus more likely to contain a recombinant plasmids
• D)all colonies will contain plasmids,but additional testing is needed to determine if the plasmids are recombinant
• E)all surviving colonies;if they grow on the media they are ampicillin-resistant and contain recombinant plasmids

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Definitions:

Middle Childhood

A developmental stage that typically includes children from the ages of 6 to 12, characterized by significant cognitive, emotional, and social growth.

Overweight

A condition characterized by exceeding the normal or healthy weight for one's height, often defined by a Body Mass Index (BMI) between 25 and 29.9.

Physical Growth

The increases in height, weight, and other body dimensions that occur as a child matures.

Middle Childhood

A developmental phase typically from ages 6 to 12, characterized by significant growth in logical thinking, self-awareness, and socio-emotional skills.