You Have the Target DNA and Primers Shown Below

question 51

Multiple Choice

You have the target DNA and primers shown below.You add the target DNA and primers,along with dATP,dTTP,dGTP,dCTP and Taq DNA polymerase to a test tube.After 30 cycles of PCR,which of the following is true about the DNA in your test tube?
Target DNA: 5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
Primer 1: 5' - GATCAA - 3'
Primer 2: 5' - CGGAAA - 3'

• A)There will be around a billion DNA molecules and most will look just like the template
• 5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
• 3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
• B)There will be around a billion DNA molecules and most will be single stranded,either
• 5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3' OR
• 3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'
• C)Only the template and primers will be present;since no DNA helicase was added the strands won't separate so no new DNA can be made
• D)There will be around a billion DNA molecules and most will contain the region that is flanked by the primers
• 5' - GATCAATCAATGCCGAATTTCCG - 3'
• 3' - CTAGTTAGTTACGGCTTAAAGGC - 5'
• E)There will be around a million DNA molecules of various sizes,depending on where chain termination occurred

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Definitions:

Predetermined Overhead Rate

A rate calculated before a period begins, used to allocate estimated overhead costs to products or services based on a chosen activity base.

Standard Activity Index

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Budgeted Overhead Costs

The estimated costs related to the indirect aspects of manufacturing or service delivery that are planned for a specific period.

Board Feet

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