Shown Below Is a Diagram for a Plasmid Vector You

question 38

Multiple Choice

Shown below is a diagram for a plasmid vector you want to use to clone a gene.The diagram shows the location of the recognition sites for four restrictions enzymes,BamHI (B) ,EcoRI (E) ,HindIII (H) ,and XhoI (X) .The genes encoding beta-lactamase (Amp^R) and beta-galactosidase (lacZ) ,which you plan to use as selectable markers,are also indicated.If you were to use this plasmid as a vector,which restriction enzyme would be most useful to prepare it for cloning?

• A)BamHI
• B)EcoRI
• C)HindIII
• D)XhoI
• E)BamHI or XhoI would be equally useful.

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Definitions:

Test Condition

A specific set of parameters or circumstances under which an experiment, test, or study is executed.

Implicit Premises

Unstated assumptions that underlie an argument, which are not expressed but are necessary for the argument's logic to hold.

Explicit Premises

Clearly stated assumptions or propositions that form the basis of an argument or reasoning.

Yogi Berra

An American professional baseball catcher, who was known for his humorous and paradoxical quotes.