You Cut Some Sheep DNA and Some Plasmid DNA with the Same

question 3

Multiple Choice

You cut some sheep DNA and some plasmid DNA with the same restriction enzyme.The plasmid contains an Amp^R gene and a lacZ gene.You mix together the fragments of sheep DNA and the linearized plasmid.You transform bacteria with your mixture,and plate the bacterial cells on growth media containing the antiobiotic ampicillin and X-gal (the substrate of beta-galactosidase) .You need to pick the bacterial colonies that contain recombinant plasmids.Which colonies do you choose?

• A)only the blue colonies - these contain recombinant plasmids
• B)only the white colonies - these contain recombinant plasmids
• C)the largest colonies - these are the most resistant to ampicillin and thus more likely to contain a recombinant plasmids
• D)all colonies will contain plasmids,but additional testing is needed to determine if the plasmids are recombinant
• E)all surviving colonies;if they grow on the media they are ampicillin-resistant and contain recombinant plasmids

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Definitions:

Nitrate

A polyatomic ion with the chemical formula NO3-, commonly found in fertilizers and an essential nutrient for plant growth.

Plants

Multicellular organisms that typically produce their own food through photosynthesis and have cell walls made of cellulose.

Uptake

The absorption or intake of substances by the cells or biological membranes.

Nutrients

Essential substances that organisms need to acquire from their environment to maintain health, growth, and reproduction.