You Have the Following DNA Template and Want to Use

question 46

Multiple Choice

You have the following DNA template and want to use PCR to amplify the double-stranded region that is underlined.What primer should you use?
5' - CCGGATCAATCAATGCCGAATTTCCGTATAGGGCCTAGTAG - 3'
3' - GGCCTAGTTAGTTACGGCTTAAAGGCATATCCCGGATCATC - 5'

• A)5' - AGGGC- 3' and 5' -GATTG- 3'
• B)5' -CAATC- 3' and 5' -GCCCT- 3'
• C)3' -CAATC- 5' and 3' -GCCCT- 5'
• D)3' - AGGGC- 5' and 3' -GATTG- 5'
• E)5' -GTTAG -3' and 5' -ATCCC -3'

---

Definitions:

Mechanism

A detailed description of the step-by-step process by which a chemical reaction occurs, including the breaking and formation of bonds.

Carboxylic Acid

Organic acids characterized by the presence of at least one carboxyl group (-COOH), widely found in nature.

Derivative

In chemistry, a compound that is derived from a similar compound by a chemical reaction.

Propyl Benzoate

A chemical compound used as a fragrance and flavoring agent, consisting of the propyl ester of benzoic acid.