You Have Made a Specific DNA Probe That Will Bind

question 51

Multiple Choice

You have made a specific DNA probe that will bind to a key sequence on the DNA of Neisseria gonorrhoeae,allowing your company to market a gonorrhea test kit that can be used to identify the bacterium in genital tract specimens.However,upon testing it out against a known Neisseria gonorrhoeae culture,you find that it does not work.Which of the following is a possible explanation for this negative result?

• A)You forgot to label the probe sequence with a reporter molecule.
• B)You forgot to digest the probe with restriction endonucleases.
• C)You forgot to add the mRNA to the DNA probe sample.
• D)You forgot to incubate the test at the 75C temperature required for hybridization.

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Definitions:

Reverse Transcriptase

An enzyme used by retroviruses to transcribe their RNA into DNA, allowing the viral DNA to be integrated into the host's genome, crucial in the replication of retroviruses including HIV.

RNA Template

A strand of RNA that serves as a guide for the synthesis of a complementary RNA or DNA molecule during transcription or replication.

Ribosome

Complex molecular machines in cells that synthesize proteins by translating messenger RNA into amino acid sequences.

Binding Sites

Specific regions on molecules (e.g., enzymes, receptors) where other molecules (e.g., substrates, ligands) can attach or bind.