If You Are Setting Up a PCR Reaction,how Much Extension

question 23

Multiple Choice

If you are setting up a PCR reaction,how much extension time per cycle should you allocate for the Taq DNA polymerase to amplify a 5-kb DNA fragment from a 10-kb template?

• A)1 minute
• B)2.5 minutes
• C)5 minutes
• D)10 minutes
• E)20 minutes

---

Definitions:

O-Rings

Circular, elastomeric seals used to prevent the passage of liquids or gases between two surfaces.

Semi-Circular

Describes a shape or object that is half of a circle or roughly forming a half-circle contour.

Load Capacity

The maximum load weight or force that a structure, machine part, or material can safely support without failure.

Roller Bearings

Bearings that use cylindrical rollers as rolling elements, offering reduced friction between rotating components.