If You Carried Out Site-Directed Mutagenesis of Subtilisin, Changing Serine

question 46

Multiple Choice

If you carried out site-directed mutagenesis of subtilisin, changing serine 221 to isoleucine, what would you expect?

• A) a large change in K_M
• B) a small change in K_M
• C) a large change in k_cat
• D) a large change in K_M and a large change in k_cat
• E) a small change in K_M and a large change in k_cat

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