Polymerase Chain Reaction (PCR) Can Be Used to Perform DNA

question 31

Multiple Choice

Polymerase chain reaction (PCR) can be used to perform DNA sequencing reactions. In this case, are 2 primers (a forward and a reverse) necessary?

• A) Yes-you can't perform PCR without 2 specific primers to amplify the region in question in the DNA.
• B) No-dideoxynucleotide sequencing depends on different length fragments being formed and then separated based on size. This can take place with only a specific forward OR a specific reverse primer.
• C) No-you actually need a primer pair for each round of DNA amplification...so you'll need many, many primer pairs.
• D) Yes-and it will be important to make sure that the primer pairs are made with dideoxynucleotides that are labeled with fluorescent dyes. Otherwise, you won't be able to detect the fragments that are made in the PCR process.

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Definitions:

Inequalities

The existence of disparities or unequal conditions in aspects such as wealth, resources, opportunities, or treatments among different groups or individuals.

Sexism

Discrimination or prejudice based on a person's sex or gender, often manifesting in societal, political, or economic disadvantages for women.

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