Cells Are Often Ground Up with Abrasive to Get to Their

question 38

Multiple Choice

Cells are often ground up with abrasive to get to their internal enzymes/molecules, followed by removal of the abrasives. Here are three situations and three outcomes. What is the correct interpretation of the results? In situation #1, radioactive amino acids are added to cell material, and radioactive proteins are produced. In situation #2, radioactive amino acids, cell material, and the enzyme DNAse (degrades DNA) are added together. Radioactive proteins are still produced. In situation #3, the ground-up cell material is allowed to sit for 24 hours before radioactive amino acids and DNAse are added to it. No radioactive protein is produced. Interpretation?

• A) RNA polymerase has no activity after 24 hours, so it is unable to transcribe any mRNA in the last scenario to be translated into radioactive proteins.
• B) The radioactivity in the amino acids is altering/degrading the tRNA molecules, leading to no protein production in the last scenario.
• C) Natural RNAses/DNAses present in the ground-up material will degrade any mRNA/DNA in that 24 hour interval. This will lead to no capability to transcribe genes/translate protein in the last scenario.
• D) The results are not interpretable from the information given.

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