The Basis of Sanger DNA Sequencing Is to

question 23

Multiple Choice

The basis of Sanger DNA sequencing is to

• A) identify ESTs and place them in order based on their function to derive the overall sequence.
• B) collect all of the mRNAs of a given cell type, make cDNAs from them, align the cDNAs, and derive the overall sequence.
• C) break several copies of a genome into 3-base codons, and align them to derive the overall sequence.
• D) generate a series of fragments from an overall DNA sequence that differ by the end base of each. Overlap the pieces and read off the overhangs to derive the sequence.
• E) tag each of the four DNA bases with a fluorescent marker molecule and align them by the intensity of the fluorescence to derive the overall sequence.

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